![]() A similar workflow can be applied to eukaryotic micro-organisms using the 18S rRNA gene. These characteristics make this gene useful for analyzing microbial communities at reduced cost compared to metagenomic techniques. It features regions that are conserved among these organisms, as well as variable regions that allow distinction among organisms. We will be following our 16S pipeline, which uses several wrapper scripts to help automate running many files.ġ6S analysis is a method of microbiome analysis (compared to shotgun metagenomics) that targets the 16S ribosomal RNA gene, as this gene is present in all prokaryotes.I'm assuming the unzipped folder will be in your Desktop. If you are running it elsewhere just be aware you will need to change the file paths. ![]()
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